Bacteriology
Modified Hodge Test (MHT)- Principle, Procedure, Results, Uses
Modified Hodge Test (MHT) is a phenotypic method for detecting carbapenemase production by isolated pathogens in clinical laboratories. It is a reliable method to confirm that the carbapenem resistance in the isolated bacterial ... Read more
Middlebrook Agar- Composition, Principle, Preparation, Results, Uses
There are many culture media that have been devised for the cultivation of mycobacteria over the years. The early ones were egg-based formulations which included Lowenstein-Jensen Medium and Petragnani Medium. Later, Dubos and ... Read more
Methicillin Resistant Staphylococcus aureus (MRSA)
MRSA is mediated by the mecA gene; which is chromosomally coded. It alters penicillin-binding protein (PBP) present on S. aureus cell membrane to PBP-2a: Types of MRSA MRSA are either community or hospital-associated. ... Read more
Martin Lewis Agar- Composition, Principle, Preparation, Results, Uses
Thayer-Martin Agar was developed in 1964 for the isolation of N. gonorrhoeae and N. meningitidis. A further modification of these early formulations was developed to improve the isolation of pathogenic Neisseria sp. from ... Read more
Mannitol Salt Agar (MSA)- Composition, Principle, Preparation, Results, Uses
Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens. It is recommended for the detection and enumeration of coagulase-positive ... Read more
Sudan Black B Staining: Principle, Procedure, Results, Uses
Sudan stain is a special stain used for staining of fats and fat droplets using several Sudan dyes, which include Sudan II, Sudan III, Sudan IV, Oil Red O, and Sudan Black B. ... Read more
MacConkey Agar- Composition, Principle, Preparation, Results, Uses
MacConkey agar was the first solid differential media to be formulated which was developed in the 20th century by Alfred Theodore MacConkey. MacConkey Agar is the earliest selective and differential medium for the cultivation of coliform ... Read more
Lowenstein Jensen (LJ) Media- Composition, Principle, Preparation, Results, Uses
Lowenstein Jensen (LJ) Media is a selective medium that is commonly used for the cultivation and isolation of Mycobacterium, specifically Mycobacterium tuberculosis from clinical specimens. LJ medium was originally formulated by Lowenstein who incorporated congo ... Read more
Transport Media: Principle, Uses, Types, Examples
Culture media are solid, semisolid, or liquid preparations containing essential nutrients and minerals vital for microorganisms’ survival and growth. They are also called growth media. They provide nutritional requirements and a place to ... Read more
TCBS Agar- Composition, Principle, Preparation, Results, Uses
Thiosulfate-citrate-bile salts-sucrose (TCBS Agar) Agar, is a type of selective agar that is used in microbiology laboratories to isolate Vibrio species. TCBS Agar is a selective differential medium for isolating and cultivating Vibrio cholerae and other ... Read more
